Mastitis4

Mastitis4 DNA Test

Milk testing for contagious Mastitis is available to both DHI and non-DHI herds. Please contact your customer service representative or call DHI at 1-800-549-4373 for more information.


The Mastitis4 DNA test identifies the presence of four mastitis pathogens commonly referred to as the 'contagious' pathogens, specifically Staph. aureus, Strep. agalactiae, Mycoplasma bovis, and Prototheca. These contagious pathogens are often the cause of chronic infections leading to ongoing elevated SCC.

Why using the Mastitis4 DNA test makes good sense:

It's Targeted: These contagious pathogens are often the cause of chronic infections leading to ongoing elevated SCC. They can spread easily from cow to cow at milking time, and for Staph. aureus and Mycoplasma bovis , are difficult to treat. Prototheca infections are not treatable.

It's Reliable: The test is based on polymerase chain reaction (PCR), which detects the presence of the bacteria’s DNA in the milk sample. You no longer have to rely on the ability of the bacteria to grow under culture conditions. The test has been validated with preserved metered samples, making it applicable to DHI services where mastitis testing has not been possible before.

It's Convenient: The regular DHI sample can be used! No more messing around with time consuming sample collection, storage and shipment.

It's Fast: Once samples are in the lab, results are usually available within a day.

It's Flexible: You have the ability to test the entire herd, selected cows (such as newly purchased or clinical cows), or cows that exceed a selected SCC level, where those samples will be redirected from the SCC analyzer to the Mastitis4 test.

It's Integrated: Positive test results, reported as +, ++, or +++ for each of the three pathogens, are displayed on an easy-to-read report and are integrated with other important SCC and DHI information for improved decision making.

General Mastitis Information

Prototheca Mastitis4 Test

Prototheca Prevention

Mastitis4 Frequently Asked Questions

Mastitis4 Factsheet

A practical look at contagious Mastitis

National Mastitis Council Website

Canadian Bovine Mastitis Research Network Website

Mastitis PCR Assay - Finnzymes Diagnostics

NMC Annual Meeting Proceedings (January, 2010)

Validation of PCR Mastitis assay for 3 major Contagious Pathogens

Field evaluation of Mastitis PCR assay with DHI sample

NMC Annual Meeting Proceedings (January, 2009)

Comparison of real-time PCR and conventional bacterial culturing in bovine Mastitis testing

Real-time PCR-based identification of bacteria in milk samples from bovine clinical Mastitis with no growth in conventional culturing

Journal of Dairy Science (March, 2009)

Analytical specificity and sensitivity of a real-time polymerase chain reaction assay for indentifcation of bovine mastitis pathogens

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